Cysteine Sulfoxidation Increases the Photostability of Red Fluorescent Proteins
نویسندگان
چکیده
منابع مشابه
Microfluidic cell sorter for use in developing red fluorescent proteins with improved photostability 3
This paper presents a novel microfluidic cytometer for mammalian cells that rapidly measures the irreversible photobleaching of red fluorescent proteins expressed within each cell and achieves high purity (.99%) selection of individual cells based on these measurements. The selection is achieved by using submillisecond timed control of a piezo-tilt mirror to steer a focused 1064-nm laser spot f...
متن کاملMicrofluidic cell sorter for use in developing red fluorescent proteins with improved photostability.
This paper presents a novel microfluidic cytometer for mammalian cells that rapidly measures the irreversible photobleaching of red fluorescent proteins expressed within each cell and achieves high purity (>99%) selection of individual cells based on these measurements. The selection is achieved by using sub-millisecond timed control of a piezo-tilt mirror to steer a focused 1064-nm laser spot ...
متن کاملImproving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting
Many genetically encoded biosensors use Förster resonance energy transfer (FRET) to dynamically report biomolecular activities. While pairs of cyan and yellow fluorescent proteins (FPs) are most commonly used as FRET partner fluorophores, respectively, green and red FPs offer distinct advantages for FRET, such as greater spectral separation, less phototoxicity, and lower autofluorescence. We pr...
متن کاملDevelopment of Cysteine-Free Fluorescent Proteins for the Oxidative Environment
Molecular imaging employing fluorescent proteins has been widely used to highlight specific reactions or processes in various fields of the life sciences. Despite extensive improvements of the fluorescent tag, this technology is still limited in the study of molecular events in the extracellular milieu. This is partly due to the presence of cysteine in the fluorescent proteins. These proteins a...
متن کاملCircular Permutation of Red Fluorescent Proteins
Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) brightness of the parent molecules are reported, as well as a quantitative evaluation of the fluor...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2016
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.6b00579